The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. * Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). 190. plates < 100 amol (384-well) 1 0200-7018. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. 96 deep well plate: packed in sleeves of 6 , 24 per case. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Supersedes 1. TNF standard curve. Made from polypropylene with conical well profiles to. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. run on the FlexStation 3 reader in 384-well microplate with the FLIPR® Calcium 4 Assay Kit. FLIPR Calcium 4 Assay Kit. 20 µL (96-well plates) or 10 µL (384-well plates. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 96-, 384-, and 1536-well microplates. 76 ± 0. A snapshot of the Settings screen is shown in Figure 2. Temperature Control: Ambient +5C up to 42C. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. x. QuickSync. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. For more sample throughput, the system can6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Rating: 3. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Recertification service. Time-resolved phosphorescence. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. x. Select the Driver tab. +1 800. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The FLEXstation II 384 (Molecular Devices, Inc. 6-384 (96- and 384-well with pipetting) 96-well microplates. 96-1536. The plate reader optics are tunable for. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. measurements in compatible Molecular Devices® microplate readers. com. flat meniscus), but is approx 15 mL. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices'. 2 with 1000 cells. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Add to Cart. [Carbachol] (nM) RFU Max. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 2. 304 signal level saturation. • 11/10 • Printed in USA • PN: 0120-1525. Detection cartridge modularity allows for easy addition of new read modes within minutes without. x. of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wavelength range (spectral scan. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 5 fg per well to 0. There is no product in the shopping cart, buy it!. Absorbance equation. What are the dimensions of the plate? Code. Infinite possibilities. This integrated system provides users with a multi-detection for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. In addition, SoftMax Pro Software After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The former have been the traditional domain of plate readers and great strides have been. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes<2 fg/well for firefly luciferase in 96- and 384- well top read. We would like to show you a description here but the site won’t allow us. Description: The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. SpectraMax ID5 Plate Reader (Molecular Devices). Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. x. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. Filter. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. x. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. 2. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. RUO. Microseal 384-Well PCR Plates. If you have an xml plate object, then. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. #6590-0118) Filter 665/10 (Molecular Devices cat. 020 fmol 0. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. It. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Recertification service. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. x. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. SpectraMax iD5. 0 mP 96-well. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. 384-well plates • Automated rinse reduces clogging • Quiet and efficient. We also prefer that fluorescence signals in one well don’t bleed into the other, as this means less background and more precise data. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. These models can no longer be repaired. Overview of 384 Well SBS. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. 1: The SPECTRAmax 340PC 384 System The on-board microprocessor calculates and reports the absorbance (or % Transmittance) for each well of a microplate. Typically, the full spectrum of signal from. 384-Well Microarray/High Sample Recovery Plate. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Option 1: Use a plate. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of. Uncontrolled Copy for Training and Reference Purposes Only . the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. ‘StimB’ is Stimulation Buffer, included in the IP-One Gq kit. Molecular Devices has provided scientists with tools to expand the boundaries of their research. Automated live cell imaging – cell counting and confluence. 9. 2. 384-Deep Square Well Plates. Programmable 10% to 100%: Sample Cell Options: 96-, 384- or 1536-well microwell plates. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Scan every well . For a higher . 2. #PLUS 384) Methods. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. #PLUS 384) UV-transparent 96-well microplates (Corning cat. After reading the ELISA plate on both readers, each standard curve was nearly identical. Catalog number: AB1384W. Temperature Control: ambient from +4 to 45C. #S1205. T = I/I 0 and %T = 100 (T). Not for use in diagnostic procedures. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer. Select Update Driver. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. 0 out of 5 $ 14,499. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Visible absorbance -. UV absorbance detection. The VICTOR Nivo. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. Key benefits • Assess cell confluence objectively and quantitatively. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 5 ml microcentrifuge tubes 35 mm Petri dishes 12 mm × 50 mm test tubes (optional). Microplates for applications that require high sample recovery rates. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Learn more about SpectraMax Plus 384 Microplate Reader >>Rating: 5. All stand-alone instrument functions can be controlled using the software. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. Reconstitution of reagents. EMax. Outer wells aligned for proper viewing with FLIPR. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Microplates, cuvettes (via adapter) Detectors. CO 2 & O 2 control. Plate chamber design of the SpectraMax category 1 microplate readers. X. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. SpectraMax i3x. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. Pfa1 and Pfa1 Gpx4-KO cells stably overexpressing murine FSP1 were seeded on separate 384-well plates (500 cells per well), and screened with a library of small-molecule inhibitor compounds, as. #3635) Transferpette micropipette (Drummond Scientific cat. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 00. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Total volume (except deep. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 8 mm distance between well bottoms to plate bottom. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). VIS absorbance detection. 100 fmol luciferase per well. These robust readers have been placed in labs from Antarctica to the. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. 0 . Cells toxicity was corrected for the culture medium background absorbance. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. $245. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Contact our support team with your questions regarding Molecular Devices products and applications. • Easy and gentle immobilization of biotin. USA. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. 96/384-well plate formats. The FilterMax F3 can read 96-well and 384-well plates. If an organoid line forms small organoids in the 24-well semi. SpectraMax® M2 and M2e Microplate Readers are the standard for UV/visible multimode reader absorbance, providing ultrafast and full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. in every plate. ,LTD (CAT#: STEM-S. x. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. . 00. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Pre-read measurements were made with 200 µL water in each well of a Costar UV plate, after which the plate was emptied and blotted dry. 012 fmol 0. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 100 fmol 1536-well (8 µL) 0. Selects either 96-well or 384-well plate for a stand-alone read. x. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. 384 only) Selects either 96-well or 384-well plate for a stand-alone read. The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. molecular interaction studies, and nucleic acid quantification, as well. 384-well format. Cell viability는 세포막 Integrity 또는. M5. White plates reflect the light, enabling even low levels of Luminescence to be detected. Bioz Stars score: 86/100, based on 1 PubMed. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 1 x plates. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Plate formats. Embla washers are durable and easy to maintain. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Figure 6. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. e. In addition, SoftMax Pro SoftwareBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Plate readers, also known as. 00. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 20 attomol ATP per well. Service parts for these models are not being produced and are no longer in stock. Integrated stacker option. 0 . Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. A = 2 - log 10 (%T). 5 ng per well. Introduction. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. com 上海 电话: 86-21-3372 1088 传真: 86-21-3372. Dual injectors for kinetic assays. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. Features. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Values are averages from 5 clean plates, each filled with 200 µL water/well. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. It is recommended that you then centrifuge the plates at 1000 rpm for up to 4 minutes (brake off). The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 384-Well Microarray/High Sample Recovery Plate. Guaranteed sensitivity: < 100 amol of biotinylated-LCK-P peptide, 25µL/well in a 384-well plate. Absorbance cuvette port. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. 0200-6263. The color touch screen provides a visual user interface, making programming fast and intuitive. Description. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. For biochemical assays, the washer removes unbound material and unreacted reagents. Microplate Readers. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Service Contracts, Preventative Maintenance and. The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. Reader SpectraMax® Paradigm® Multi-Mode Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). x. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Figure 2-23: Bottle holder To install the bottle holder: 1. Expanded wavelength coverageIn the software select Tools > Configuration. 2. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Expand the Microplates tree. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. This can increase throughput and efficiency, thus saving time and space in the lab. For biochemical assays, the washer removes unbound material and unreacted reagents. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. For nearly 30 years, Molecular Devices has provided scientists with . If the computer is connected to the Internet: Select Search Automatically for. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Documentation:Molecular Devices microplate readers. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. M2. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. 58% for the detection module and 2. The drawing shows a standard microplate of 15 mm height covered with a lid. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. XML file. Top. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Artel MVS Bundle DM Std/800TSNB Reader Details. 1. Plate reader instructions Computer. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Alpha 384 HTS 96- and 384-well . For more sample throughput, both readers can be easily. 384 well polystyrene microplates have plate dimensions of 127. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. SpectraMax ID5 Plate Reader (Molecular Devices). x. 984. Inquiry Basket. 384- or 96-well read plate containing cells loaded. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (T onset, T m, T agg) and colloidal stability (A 2 /B 22,. 5. 1. both 96- and 384-well plates and comes with four wash/rinse bottles and one waste. (96- and 384-well with pipetting) 6-384. Compare specifications and request a quote from leading suppliers. 340 PC384. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Experience the identifiably different. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 96-, 384-, and 1536-well microplates. Microplate Formats: 6, 12, 24, 48, 96, 384. Loading Buffer was also added to a set of cell-free wells for background controls. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 6-, 12-, 24-, 48-, 96-, and 384-well microplates; Extended research windows available; Temperature control; Returnable samples; Repeatable experiments;The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument.